16S/ITS Amplicon Sequencing

16S rRNA gene encodes 16S ribosomal RNA and is present in all bacteria. The gene sequences contain several hypervariable regions that can accommodate species-specific signature sequences useful for identification of bacteria. 16S rRNA gene sequencing is a common and well-established amplicon sequencing method for studying phylogeny and taxonomy of samples from complex microbiomes or environments that are challenging to study. The internal transcribed spacers (ITS) region is the most widely sequenced DNA region in molecular ecology of eukaryotes and has been recommended as the universal fungal barcode sequence. There are two ITSs in eukaryotes, ITS1 is located between 18S and 5.8S rRNA genes, while ITS2 is between 5.8S and 26S rRNA genes. The ITS region is widely used in taxonomy and molecular phylogeny because of high degree of variation even between closely related species.

Sequencing platform and strategy

Illumina HiSeq 2500 platform; Paired-end 250 bp

Technical route
Analysis content and analysis chart display (partial)

Analysis content

一、Species annotation and evaluation

二、Species classification and abundance analysis

三、Alpha-diversity analysis

四、Beta-diversity analysis

五、Significance differences analysis

六、Advanced Analysis

七、Personalized analysis

  • Correlation analysis

  • Correlation analysis of the top 30 species

  • Boxplot of Differential OTUs

  • LefSe analysis

Technical advantages
  • Rich project experience

    high output of papers

  • Mature experimental technology

    reasonable analytical methods

  • Comprehensive analysis content

    keep up with international frontier

  • Exquisite pictures presentation

    highly favored by mainstream journals